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Water was freely available for both SLD- and HFD-fed mice. The mice were kept on either the SLD or the HFD for a period of 12 weeks during which time food intake was measured. The weight of the mice was monitored weekly. Blood was collected from a subset of these mice at the 12 week point, for determination of plasma glucose and insulin concentration. C57/BL6 mice were humanely killed via CO2 inha